Antagonıst Test of Endophyte Fungi Isolated from Leaves of Mangrove (Rhizophora sp.) as Antıfungi against Sanca Snakes (Malayopython sp.) Disease

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Dalia Sukmawati, Atin Supiyani, Rahmah Khairunnisa Qonita, Clarita Salsabila, Rila Nazila Herlambang, Riska Ariska, Andrian Bayu Sentosa, Desty Saszieta, Proborini Indah Nursari, Almira M. Priskaningrum, Famira Ichsanty, Sheyla Annisyah, Hesham Ali El Enshasy, Daniel Joe Dailin, Skyler, Low Hock Heng, Raden Haryo Bimo Setiarto, Sulistiani, Dandy Yusuf, Lutfi Anshory, Ahmad Fathoni

2024 Current Applied Science and Technology Vol. 24 Issue 2 Article Cited by 1 SDG 15SDG 17 Quartile

Abstract

Mangrove plants have many benefits, such as the ability to produce bioactive compounds. Bioactive compounds can be produced by endophytic fungi found on mangrove leaves. Endophytic fungi are also known to produce secondary metabolites that are the same as their hosts produce for plant defence. Endophytic fungi in mangrove leaves (Rhizophora sp.) have antagonistic potential against pathogenic fungi. One of the pathogenic fungi was shown to cause a skin disease in pythons (Malayopython sp.) through the transmission via tick saliva. Pathogenic fungi found in tick saliva were Fusarium oxysporum and Aspergillus niger. This study examined the potential of endophytic fungi in mangrove leaves (Rhizophora sp.) as anti-fungal pathogens in pythons. Endophytic fungi were isolated from mangrove leaves at the Angke Kapuk Mangrove Nature Park, Pantai Indah Kapuk, DKI Jakarta, and an antagonist test of endophytic fungi against pathogenic fungi was performed using the dual culture assay method. This study obtained 16 isolates, and nine isolates had antagonistic potential. These nine isolates produced a clear zone in the antagonist test. The inhibition zone indicates endophytic fungi inhibit the growth of pathogenic fungi. © 2024, King Mongkut's Institute of Technology Ladkrabang. All rights reserved.

Affiliations

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jl. Rawamangun Muka, East Jakarta, Indonesia; Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Skudai, Johor Bahru, Malaysia; Malaysia1Institute of Bioproduct Development, Universiti Teknologi Malaysia, Johor, Skudai, 81310, Malaysia; Sateera Biotech Sdn. Bhd, Malaysia; Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN), Cibinong Science Center, Jalan Raya Jakarta-Bogor Km 46, Cibinong, West Java, Bogor, 16911, Indonesia

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