Yussi Pratiwi, Elsa Vera Nanda, Aulia Natasya
Analysis of phosphore using UV-Vis Spectrophotometry has been studied. The aims of this research were to get the optimum conditions for analysis phosphore, to obtain information on the best reductor, and to apply this method for analysis phosphore in vegetables that reach method validation parameters. This research begins with determination of optimum conditions of phosphorus analysis by forming a yellow complex compound. This compound was unstable so it needs to be reduced using SnCl2 and thiourea with various acid conditions to produce a stable blue complex. Furthermore, the phosphorus analysis method was validated based on the method validation parameters. Molybdenum blue reaction produced a heterophosphomolybdate complex compound which has a maximum wavelenght 689 - 729 nm. The method validation results of phosphomolybdate have linierity (R2) were 0.9983 and 0.9961 for SnCl2 and thiourea as reductors. The molar absorptivity were 2.093 x 105 L mol-1 cm-1 and 2.93 x 105 L mol-1 cm-1 for SnCl2 and thiourea. It had limit of detection and limit of quantification of 1.41 x 10-3 mg L-1 and 4.28 x 10-3 mg L-1 for SnCl2 and 5.62 x 10-4 mg L-1 and 1.7 x 10-3 mg L-1 for thiourea. The %RSD values in each sample ranged from 1.30 to 1.51%. %recovery obtained in each sample ranged from 93.02% - 110.52%. The phosphates contained in the vegetables samples did not pass the food quality standard according to the Indonesian Nutritionist Association (PERSAGI). © 2024 American Institute of Physics Inc.. All rights reserved.
Chemistry Study Progam, Fakulty of Mathematics and Natural Science, Universitas Negeri Jakarta, Jakarta, 13220, Indonesia
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