M. Nurjayadi, G.I. Putri, J.L. Declan, I. Krisdawati, D.A. Juliansyah, M. Azzahra, I. Maulana, I.R. Kartika, F. Kurniadewi, N.K. Liman, T. Fahriza, D. Sukmawati, S. Rahayu, V. Saamia, D.A.O. Saputro, I.M. Wiranatha, H.E. Enshasy
The case of foodborne diseases has now become a serious global problem. Foodborne diseases occur due to the contamination of pathogenic bacteria. One of the pathogenic bacteria that cause foodborne diseases is Yersinia enterocolitica. This research aimed to determine the annealing temperature of Yersinia enterocolitica ymoA gene primers using the PCR method to ensure that the primers can amplify ymoA gene fragments in Yersinia enterocolitica. In this research, the concentration of DNA as a template used was 70.5 ng/µL with a purity of A260/280 1.88. Optimization of the annealing temperature of Yersinia enterocolitica ymoA gene primers using the Gradient PCR in a range of temperatures between 53ºC - 62ºC based on the melting temperature value of the ymoA primers. The results of the primers design of the ymoA gene were confirmed to amplify the DNA fragment of Yersinia enterocolitica with an amplicon length of 185 bp. Based on the results, the temperature of 60oC was chosen as the optimum primer annealing temperature for the ymoA gene in Yersinia enterocolitica because only a brightness band was produced from the electrophoresis. This result can be continued with primers testing the ymoA gene on meat samples using the Real-Time PCR method. © 2024 American Institute of Physics Inc.. All rights reserved.
Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Gedung KH. Hasjim Asj'ari, 6th Floor, Jl. Rawamangun Muka, Jakarta Timur, 13220, Indonesia; Research Center for Detection of Pathogenic Bacteria, Lembaga Penelitian dan Pengabdian Kepada Masyarakat, Universitas Negeri Jakarta, Jl. Rawamangun Muka, Jakarta Timur, 13220, Indonesia; Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Building KH. Hasjim Asj'ari, 9th Floor, Rawamangun Muka Street, East Jakarta, 13220, Indonesia; Center Forensic Laboratory of the Criminal Investigation, Police of the Republic of Indonesia, Ciapmbuan Babakan Madang, Bogor, 1681, Indonesia; Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), Johor Barhru, Skudai, Malaysia; School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Johor Bharu, Skudai, Malaysia; City of Scientific Research and Technology Applications, New Burg Al Arab, Alexandria, Egypt
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