Tri Handayani Kurniati, Noer Syahbani, Sri Rahayu
Exopolysaccharides (EPS) is defined as biopolymers of long chains of polysaccharides composed of sugar units and their derivatives and will be secreted outside the cell. EPS can be widely used in various industrial fields. This study aims to obtain isolates of EPS-producing bacteria from pickled fruit and determine their identity based on 16S rRNA sequence analysis. Based on screening results on MRSA media enriched with 9% skim milk for 48 hours at 33oC, A total of 15 bacterial isolates from certain pickled fruit with various morphological characteristics were able to produce EPS. Three bacterial isolates, A4, M1, and P4, were known to have an EPS index value of 0.43, 0.33, and 0.30 and also had a relatively fast growth compared to other isolates. Analysis of 16S rRNA sequences showed that the isolates A1, M1, and P4 from pickled fruit were identified as Peribacillus acanthi A4 (99,69%), Priestia flexus M1 (99,15%), and Priestia megaterium P4 (99,77%). The results ability assay to produce EPS showed that Peribacillus acanthi A4, Priestia flexus M1, and Priestia megaterium P4 could produce EPS (ppm) sequentially as much as 847.00; 1,013.57; and 1,007.29. The results of the analysis variance revealed that the capability of EPS production of each tested bacteria was found to be statistically significant (P<0.05). DMRT 5% Revealed there was no statistically significant capability of EPS production between Priestia flexus M1 and Priestia megaterium P4. The results of this study provide information on EPS-producing bacteria from pickled fruit that can be developed as bio-thickener, bioremediation, and immunomodulatory agents. © 2024 American Institute of Physics Inc.. All rights reserved.
Biology Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jl. Rawamangun Muka, Rawamangun, Jakarta, 13220, Indonesia
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