Shanmugaprakasham Selvamani, Solleh Ramli, Daniel Joe Dailin, Khairun Hani Natasya, Theodoros Varzakas, Bassam Abomoelak, Dalia Sukmawati, Muktiningsih Nurjayadi, Siqing Liu, Vijai Kumar Gupta, Hesham Ali El Enshasy
This study reports on a novel technique to enhance the high cell mass and viable cell counts of the heterofermentative probiotic strain, Limosilactobacillus reuteri. This is the first report on the cultivation of L. reuteri, which was incorporated with weak base anion-exchange resins to remove the accumulating lactic acid in the fermentation broth. Two anion-exchange resins—Amberlite IRA 67 and IRA 96—were found to have a high adsorption capacity with lactic acid. Batch fermentation and fed-batch cultivation were further analyzed using IRA 67 resins, as this application resulted in a higher maximum number of viable cells. The in situ application of anion-exchange resins was found to create shear stress, and thus, it does not promote growth of L. reuteri; therefore, an external and integrated resin column system was proposed. The viable cell count from batch fermentation, when incorporated with the integrated resin column, was improved by 71 times (3.89 × 1011 ± 0.07 CFU mL−1) compared with control batch fermentation (5.35 × 109 ± 0.32 CFU mL−1), without the addition of resins. The growth improvement was achieved due to the high adsorption rate of lactic acid, which was recorded by the integrated IRA 67 resin system, and coupled with the stirred tank bioreactor batch fermentation process. © 2022 by the authors.
Institute of Bioproduct Development, Universiti Teknologi Malaysia (UTM, Skudai, 81310, Malaysia; School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Skudai, 81310, Malaysia; Nutrition Technologies Sdn. Bhd, No 1 No 3, Jalan Silc 2, Kawasan Perindustrian SiLC, Nusajaya, 79150, Malaysia; Department Food Science and Technology, University of the Peloponnese, Kalamata, 24100, Greece; Arnold Palmer Hospital Paediatric Speciality Diagnostic Laboratory, Orlando, 32806, FL, United States; Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Timur, Jakarta, 13220, Indonesia; Agriculture Research Service, Renewable Product Technology Research Unit, National Center for Agricultural Utilization Research, USDA, 1815 N University St, Peoria, 61604, IL, United States; Biorefining and Advanced Materials Research Center, SRUC, Kings Buildings, West Mains Road, Edinburgh, EH9 3JG, United Kingdom; Center for Safe and Improved Food, SRUC, Kings Buildings, West Mains Road, Edinburgh, EH9 3JG, United Kingdom; City of Scientific Research and Technology Applications, New Burg Al Arab, Alexandria, 21934, Egypt
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