Neneng Ambarwati, Berna Elya, Amarila Malik, Hanita Omar, Muhammad Hanafi, Islamudin Ahmad
Isolation and determination of antibacterial compounds from plants are essential to obtain a new antibacterial as a substitute for conventional resistant antibiotics. This study aims to isolate and identify a new robustaflavone as antibacterial activity from Garcinia latissima Miq. leave. In this study, the isolation process was carried out using column chromatography followed by preparative thin layer chromatography (TLC) based on the TLC profile. The fraction D was tested for anti-bacterial Bacillus subtilis using the TLC bioautography method. The isolates obtained were then identified using 1H-NMR, 13C-NMR, distortionless enhancement by polarization transfer, heteronuclear single quantum coherence, and heteronuclear multiple bond coherence. The Activity assay of the isolate was performed using the microdilution method. A pure compound obtained the result of the separation process with eluent n-hexane: Ethyl acetate (3:2) with R f 0.6. This spot follows the spot in the contact bioautographic result of fraction D, the spot with R f 0.6 gives an inhibition zone. After identifying and purifying the isolate were known as Robustaflavone, this compound has activity against B. subtilis with a (minimum inhibitory concentration) value of 2500 ppm. Robustaflavone successfully isolated and identified from G. latissima leave and its antibacterial activity. © 2022 Wolters Kluwer Medknow Publications. All rights reserved.
Department of Cosmetology, Faculty of Engineering, Universitas Negeri Jakarta, East Jakarta, Indonesia; Pharmacognosy-Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia; Microbiologi-Biotechnology, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia; Centre of Foundation Studies in Science, Chemistry Division, University of Malaya, Kuala Lumpur, Malaysia; Research Centre for Chemistry, Indonesian Institute of Sciences, South Tangerang, Banten, Indonesia; Department of Pharmaceutical Sciences, Faculty of Pharmacy, Pancasila University, South Jakarta, Jakarta, Indonesia; Department of Pharmaceutical Sciences, Pharmaceutical Research and Development Laboratory of FARMAKA TROPIS, Faculty of Pharmacy, Universitas Mulawarman, East Kalimantan 2nd Floor, Jalan Kesehatan, Samarinda, 75119, Indonesia
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