Wahyu Widowati, Meilinah Hidayat, Ahmad Faried, Fanny Rahardja, Rimonta Febby Gunanegara, Dwi Nur Triharsiwi, Rajwa Dzakiyyah Basyasyah, Rizal Azis, Didik Priyandoko, Dhanar Septyawan Hadiprasetyo, Kasim Sakran Abass
Background: Burns are a major global health problem, often leading to prolonged inflammation and impaired wound healing. Current treatments face limitations, and human Wharton’s Jelly-derived mesenchymal stem cell (hWJ-MSCs) secretome offers a promising non-cellular alternative. Freeze-dried secretome gel (FDSG) preserves bioactive molecules that promote tissue repair. This study evaluated the potential of FDSG for burn wound healing in a rat model. Methods: The secretome was obtained as a conditioned medium from hWJ-MSCs. FDSG was made by mixing 6 g of carbomer gel with 6 mL hWJ-MSCs secretome, then followed by lyophilization. An in vivo test was conducted on six Sprague-Dawley rats with a third-degree burn model (BM) with various treatments. Burn healing was evaluated on days 3, 10, and 24 based on wound reduction, IL-1β protein levels (Immunohistochemistry), and collagen density (Masson’s trichrome staining). Results: FDSG increased the percentage of wound reduction and collagen density from day 3 to 24. Our findings also indicate a regulatory pattern of IL-1β protein levels throughout the biological mechanism of tissue repair, which is strongly associated with the distinct phases of healing. The treatment group that applied FDSG twice daily demonstrated the highest effectiveness. Conclusion: FDSG shows promising results as an effective wound healing agent. © 2026 Jordan Journal of Biological Sciences. All rights reserved.
Faculty of Medicine, Maranatha Christian University, Bandung, Indonesia; Department of Neurosurgery, Mayapada Hospital Bandung, Indonesia; Department of Neurosurgery, Faculty of Medicine, Padjadjaran University Hospital, Jatinangor, Indonesia; Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, Indonesia; Faculty of Mathematics and Natural Sciences Education, Universitas Negeri Jakarta, Jakarta, Indonesia; Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, Indonesia; Faculty of Mathematics and Natural Sciences Education, Universitas Pendidikan Indonesia, Bandung, Indonesia; Faculty of Pharmacy, Universitas Jenderal Achmad Yani, Cimahi, Indonesia; Department of Physiology, Biochemistry, and Pharmacology, College of Veterinary Medicine, University of Kirkuk, Kirkuk, Iraq
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