Muktiningsih Nurjayadi, Royna Rahma Musie, Anisa Fitriyanti, Jefferson Lynford Declan, Novitasari, Bassam Abomoelak
Proteus mirabilis is a pathogenic bacterium that can cause gastrointestinal infections, bacteremia, and Urinary Tract Infections (UTI). Therefore, it is necessary to have a fast, sensitive, specific, and accurate detection method to identify Proteus mirabilis. This study aims to determine the confirmation, specificity, and sensitivity of the hpmA gene primer to detect Proteus mirabilis swiftly and precisely using the real-time Polymerase Chain Reaction method. Gradient Polymerase Chain Reaction results showed the hpmA primer has an amplicon length of 195 bp and an optimum annealing temperature at 60°C. The primer pair produced a Ct value of 10.40±0.18 and showed one peak in the melting curve with a Tm value of 81.84°C±0.02 by real-time PCR. In addition, the hpmA primer can distinguish target and non-target bacteria based on the variation in Ct and Tm values formed. Based on these results, the concentration of bacterial DNA detected by primers reached 3.2 pg/μL, equivalent to the concentration of target bacteria that can be detected by primers, which is 10.24×102 CFU. In the next step, the hmpA primer will be developed to detect Proteus mirabilis in artificially contaminated samples using real-time PCR. © 2025, Universitas Negeri Semarang. All rights reserved.
Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Gedung KH. Hasjim Asj’ari, 6th Floor, Jl. Rawamangun Muka, Jakarta Timur, 13220, Indonesia; Research Center for Detection of Pathogenic Bacteria, Lembaga Penelitian dan Pengabdian Kepada Masyarakat, Universitas Negeri Jakarta, Jl. Rawamangun Muka, Jakarta Timur, 13220, Indonesia; Research Center for Testing Technology and Standard, National Research and Innovation Agency (BRIN), Jl. Raya Puspitek Serpong, Tangerang Selatan, 15314, Indonesia; Arnold Palmer Hospital Pediatric Specialty Diagnostic Laboratory, Orlando, 32806, FL, United States
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