Rini Puspitaningrum, Risma Dwi Untarf, Amien Ramadhan Ishak, Mella Ferania, Trimurtiati, Chris Adhiyanto, Takenori Nitta, A.B. Susanto, Ria Amelia, Yasuhiro Yamashiro, Hattori Yukio
Molecular information of type 2 diabetes mellitus (DMT2) patients in Javanese tribe was still not available. One of the factors contributing in this disease is genetic. Point mutations in mitochondrial DNA are associated with the condition of DMT2. This study aimed to identify the A3243G mutation of tRNA gene in DMT2 Javanese patients from Wates Hospital at Yogyakarta, Indonesia. This descriptive study was done in laboratory with PCR and sequencing. From 160 patients in one month in Wates Hospital (Yogyakarta, Indonesia), was chosen randomly to get 100 patients. From 100 patients that were done with DNA isolation, there was 20 DNA samples in the best condition. This research was conducted on March to June 2012 at laboratory of biochemistry FMIPA Universitas Negeri Jakarta and laboratory of Clinical Technology, Faculty of Health Science, Yamaguchi University School of Medicine of 20 DMT2 Javanese patient samples, there was no mutation in a tRNA gene. This research has been successfully identified the nucleotide of the tRNA gene fragment in 20 DMT2 Javanese patients in Yogyakarta and there was no mutation in the tRNA gene. © 2015 Global Science Publications.
Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Indonesia; Faculty of Health Sciences, Yamaguchi University, School of Medicine, 1.1.1 Minami Kogushi, Ube, Yamaguchi, 755-850, Japan; Faculty of Medicine and Health Science, Syarif Hidayatullah Islamic State University, Indonesia; Faculty of Fisheries and Marine Sciences, University Diponegoro, Semarang, Indonesia; Program Immunology, Graduate Program, Airlangga University, Surabaya, Indonesia
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