Sana Shabbir, Dalia Sukmawati, Irfan Ahmad, Hafiz Abdullah Shakir, Muhammad Khan, Marcelo Franco, Muhammad Irfan
In this study, seed pods of Heterophragma adenophyllum were used as a cheap feedstock for bioethanol production. The substrate was pretreated chemically (different concentrations of alkali (KOH and NaOH) and acids (H2SO4and HCl), and thermochemically. Results revealed that alkali treatment yielded maximum exposure of cellulosic content (56%) at the concentration of 1.5%. The pretreated substrate, having maximum cellulose content was further utilized in separate hydrolysis and fermentation, and simultaneous saccharification and fermentation using Saccharomyces cerevisiae, along with seven different bacterial strains, such as Bacillus paralichniformis, Bacillus megaterium, Bascillus flexus, Bascillus wiedmanni, Pseudomonas stutzeri, Bascillus aerius and Bascillus subtilis. The results of both fermentation techniques showed that separate hydrolysis and fermentation demonstrated a higher yield of bioethanol (6.43%) than SSF (5.08%). These results suggested that seed pods of H. adenophyllum could be utilized as a potential feedstock for large-scale ethanol production. © 2025, Publishing House of the Romanian Academy. All rights reserved.
Department of Biotechnology, University of Sargodha, Punjab, Sargodha, Pakistan; Department of Biology, Faculty of Mathematics and Natural Science, University Negeri Jakarta, Jakarta, Indonesia; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia; Institute of Zoology, University of Punjab, New Campus, Lahore, 54590, Pakistan; Department of Exact Science, State University of Santa Cruz, Bahia, Ilheus, Brazil
Research at a Glance
Register to unlock
Topics & SDG Alignment
Register to unlock
Collaboration
Register to unlock
Author Profile (Selected)
Register to unlock
References Overview
Register to unlock
Journal & Source
Register to unlock
Metadata & Integrity
Register to unlock