Nia Kania, Eka Yudha Rahman, Didik Priyandoko, Adilah Hafizha Nur Sabrina, Wahyu Widowati, Rizal Azis, Aziz Annaba, Dhanar Septyawan Hadiprasetyo, Garry Alexandro
The prevalence of prostate cancer cases in men is expected to continue increase. In 2040, it is estimated that there will be 2.293.818 new cases and a 1.05% increase in the death rate due to prostate cancer. Eurycoma longifolia Jack roots extract (ELE) has potential as an alternative treatment. This study aims to analyze ELE potential as an anti-prostate cancer agent through in silico assay and in vitro assays on the prostate cancer cell line (PC-3). ELE compounds were docked against Casp-3, Casp-8, HAX-1, p27, and PTEN. In vitro assays on PC-3 cells were used, namely cell viability (WST-8), ROS levels; cell cycle; and cell apoptosis (flow cytometry), PC-3 cell senescence ( β-Galactosidase staining), Casp-3; Casp-8; HAX-1; p27; and PTEN gene expression (qRT-PCR). All proteins target were successfully docked with ELE compounds and presented binding interactions. ELE is known to reduce viability, intracellular ROS levels, live cells, necrosis, and reduce HAX-1 gene expression, and inhibit the cell cycle G0/G1 phase. ELE can also increase inhibition, senescence, late and early apoptosis, and Casp-3, Casp-8, p27, and PTEN gene expression. ELE 100 μg/mL is the most effective concentration. ELE has potential as an anti-prostate cancer agent through apoptosis, cell cycle, and antioxidant pathways. © 2025, Magister Program of Material Sciences, Graduate School of Sriwijaya University. All rights reserved.
Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, 70123, Indonesia; Biology Study Program, Faculty of Mathematics and Natural Sciences Education, Universitas Pendidikan Indonesia, Bandung, 40154, Indonesia; Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, 40163, Indonesia; Faculty of Medicine, Maranatha Christian University, Bandung, 40164, Indonesia; Biomedical Engineering Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, 16424, Indonesia; Faculty of Pharmacy, Universitas Jenderal Achmad Yani, Cimahi, 40525, Indonesia; Department of Biology, Faculty of Mathematics and Science Education, Jakarta State University, East Jakarta, 13220, Indonesia
Research at a Glance
Register to unlock
Topics & SDG Alignment
Register to unlock
Collaboration
Register to unlock
Author Profile (Selected)
Register to unlock
References Overview
Register to unlock
Journal & Source
Register to unlock
Metadata & Integrity
Register to unlock